July06, 2022,UK


Abstract Volume: 5 Issue: 2 ISSN:

Demineralization, White Spot Lesions & Remineralization During and After Orthodontic Treatment - A Narrative Review

Dr. Jyoti Yadav *1, Dr. Amanish Singh Shinh 2, Dr. Pritesh Singla3, Dr. Amanpreet Singh Natt4, Dr. Karan Maheshwari 5, Dr. Sharnjeet Kaur 6.


1. Second Year Post graduate, Department Orthodontics and Dentofacial Orthopedics, Adesh Institute of Dental Sciences and Research.

2. Principal and Head of the Department, Department Orthodontics and Dentofacial Orthopedics, Adesh Institute of Dental Sciences and Research.

3,4. Professor, Department Orthodontics and Dentofacial Orthopedics, Adesh Institute of Dental Sciences and Research.

5. Assistant Professor, Department Orthodontics and Dentofacial Orthopedics, Adesh Institute of Dental Sciences and Research.

6. Senior Lecturer, Department of Orthodontics and Dentofacial Orthopedics, Adesh Institute of Dental Sciences and Research.

Corresponding Author: Dr. Jyoti Yadav, Second Year Post graduate, Department Orthodontics and Dentofacial Orthopedics, Adesh Institute of Dental Sciences and Research.

Copy Right: © 2022 Dr. Jyoti Yadav, This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received Date: March 17, 2022

Published Date: April 01, 2022


Oral hygiene becomes cumbersome in patients with fixed orthodontic appliances and there is prevalent decalcification of the enamel surface adjacent to these bonded attachments. Decalcification is described as a white spot lesion (WSL) that orthodontic patients develop more than non-orthodontic patients. WSLs may produce carious cavitations if left untreated, and may also present esthetic problems. White spot lesions affect the esthetics of smile and the treatment of WSLs is important to decrease tooth decay and discoloration.

Key words- Demineralization, Remineralization, Oral hygiene, fixed orthodontic appliances, white spot lesions.

Demineralization, White Spot Lesions & Remineralization During and After Orthodontic Treatment - A Narrative Review


Pearly white enamel of human dentition add all the glamour to smile. Any discolouration or spotting on teeth can have psychological impact on patient which prevents him or her from a social smile. Demineralization is a major challenge in orthodontics which appears as white spot lesions of enamel, around fixed orthodontic appliances. (1) Enamel decalcification or white spot formation can occur whenever bacterial plaque is retained on the enamel surface for a prolonged period.2 Oral hygiene and oral prophylaxis are more difficult with fixed appliances. These appliances create stagnant areas where accumulation of plaque  increases, which causes enamel demineralization .The prevalence of these white spot lesions arising during fixed appliance therapy was widely from 2% to 96%.s. A recent study showed that up to 97 per cent of the patients treated with fixed appliances had WSL after therapy (Boersma et al., 2005) Orthodontic patients have an increased risk of developing gingivitis and enamel decalcifications that can lead to white spots and caries. (3,4,5,1,6)

Since fixed orthodontic appliances were introduced, WSLs have become a particular clinical problem. These can be attributed to the difficulties in performing oral hygiene procedures on bonded brackets and bands and the prolonged plaque accumulation on tooth surfaces.7,8 Recently a clinical study showed that 50% of the subjects with no preventive fluoride programs experienced an increase of WSLs during treatment with fixed appliance therapy. (9)



White spot lesions (WSLs) are defined as a “subsurface enamel porosity from carious demineralization” that presents as “a milky white opacity when located on smooth surfaces.’’10 (Samir E bishara). The formation of WSLs is due to inadequate maintenance of oral hygiene and the extended accumulation of plaque that remains on the teeth. The enamel demineralization is a challenge in orthodontics, and the presence of these lesions significantly affects the esthetic appearance of the teeth and quality of life11

These white spot lesions are due to demineralization of the enamel by organic acids produced by cariogenic bacteria. These bacteria release acid by-products, lowering the pH of the plaque. As the pH drops below the verge for remineralization, carious decalcification takes place. (12,13)

The levels of acidogenic bacteria, such as S. mutans, become significantly elevated in orthodontic patients. The first clinical evidence of this demineralization is visualized within a time frame of 4 weeks, which is usually within the time period between two orthodontic visits. These WSLs are a significant finding and are important for both the patient and the clinician to realize. (13,14)

The white appearance of initial carious lesions is because of an optical phenomenon which is caused by loss of minerals in the subsurface enamel. There is substantial evidence that the initial mineral loss results in the development of a carious lesion at the surface of the enamel. Such lesions are called surface softened lesions and in the presence of fluoride in the oral environment they may develop into subsurface lesions. (15)

Dental tissues are continuously undergoing cycles of demineralization and remineralisation. A change in pH of the oral cavity results in demineralization, which can lead to loss of  tooth surface. A reversal can occur if pH increases, resulting in deposition of calcium, phosphate, fluoride and other agents . For these reasons, tooth brushing with a multivalent toothpaste looks like the most convenient method  for plaque control and salivary mineral resources acquisition. (16)

Prevention of  WSLs

How to treat WSLs after removal of fixed appliances to produce a sound and esthetic  enamel surface is a question yet to be fully answered. Various treatment protocols and products have been suggested, such as remineralization through saliva, mouth rinses, and toothpastes with various components such as increased fluoride concentrations, casein phosphopeptide amorphous calcium phosphate, nanohydroxyapatite and calcium sodium phosphosilicate glass. To prevent decalcification and formation of white spot lesions, a good oral-hygiene regimen must be implemented, including proper toothbrushing with a fluoridated dentifrice (17,9)

WSLs should be managed using a multifactorial approach. The most important mechanism to arrest these lesions and biofilm formation, and use of strategies to prevent remineralization of these lesions,weakening of enamel, microabrasion, erosion?infiltration, adhesives, and the bonded facets. (18)

I. Oral hygiene control

Prevention should begin by tutoring and prompting the patient for maintaining good oral hygiene and  compliance with a noncariogenic diet. Motivation includes professional instructions, adequate tools, and patient education. Fruitful oral hygiene is the substructure of prophylactic measures in orthodontic patients. Removal of plaque biofilm by proper brushing of the teeth twice daily with fluoride dentrifrices is highly adviced. (19,20)

Studies have proved that it takes minimum of 6 months for caries to develop, in a patient not undergoing orthodontic treatment, whlile it takes approximately 1 month to develop in an orthodontic patient because of the difficulty these patients have with performing oral hygiene (21)

II. Remineralizing Fluorinated Agents

• Fluoridated toothpaste

It has been well established that the application of fluoride to newly formed enamel white spot lesions will promote remineralization. The most commonly employed fluoride-containing formulations, such as dentifrices, contain sodium fluoride.22 Fluoride is important in the prevention of enamel demineralization. (23) The fluoride concentration of toothpastes above 1000ppm is recommended. The use of toothpaste with a high fluoride concentration (5000 ppm), twice daily, by patients at high risk for enamel decalcification is more effective than traditonal practices. (24)

• Mouthwashes containing fluoride

Daily use of mouthwashes containing NaF has showed marked decrease in the development of WSLs around bands and brackets. A regime containing daily mouthwash with concentration of NaF (0.05% or 0.2%) and/or weekly rinse containing alpha?1?fetoprotein (1.3%) have been demonstrated to minimize the incidence of enamel demineralization during fixed mechanotherapy.


III. Non-Fluoride Remineralizing Agents

The use of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) has been described to induce remineralization because of its potential to balance calcium and phosphate in an unstructured state, hindering the accretion of calcium phosphate to a pedantic aggregate that leads to precipitation3

Decalcification of enamel by products incorporating (CPP?ACP), which was assimilated through the enamel surface and contrived the demineralization?remineralization cycle. Reynolds reported that CPP?ACP, is derived from milk casein. (25,26) Present day research has shown that this is accomplished by a part of the casein protein  as which trasport calcium and phosphate ions “held” to it, in the form of APP. Reports have proved  that the anticariogenic activity of CPP?ACP depends on the aggregate of nanocomplexes into the dental plaque and on the tooth surface, thereby behaving as a calcium and phosphate reservoir. CPP?ACP binds to the bacterial wall and tooth surfaces. (27,26)

IV. Probiotics

Probiotics are live microorganisms that might enhance the effect of fluoride in preventing dental caries. They provide health benefits when they are dispensed in sufficient numbers. It is postulated that probiotic strains impede with other microorganisms, particularly pathogens.


V. Polyols

Polyols are sweeteners that are not metabolized by caries producing bacteria or weakly metabolized (sorbitol). Substanital reports state that xylitol is noncariogenic,which manifest as a dose? and frequency?dependent effect on dental plaque and mutans streptococci. Chewing gum with xylitol (2 g of xylitol/socket) is prescribed after each meal (three times daily) for 10?20 min.

VI. Nano hydroxyapatite

To enhance lesion remineralization, increase of calcium or fluoride concentrations in the oral fluids would seem reasonable20Nano-hydroxyapatite (n-HAp) is considered one of the most biocompatible and bioactive materials, and has gained wide acceptance in medicine and dentistry in recent years. Synthesis of nano-scaled zinc carbonate hydroxyapatite (ZnCO3/n-HAp) yielded a significant progress, and showed considerable affinity to the enamel surface. Nano-sized particles have similarity to the apatite crystals of tooth enamel in morphology and crystal structure. (28)

VII. Enamel Remineralizing Systems

These agents can be categorized into one of three types—crystalline, unstabilize amorphous, or stabilized amorphous formulations. (29)


Unstabilized ACP Systems

The (ACP) technology is an unstabilized calcium and phosphate system that has been evolved and advertised. Its technique is based on unstabilized ACP, where a calcium salt (e.g., calcium sulphate) and a phosphate salt (e.g., potassium phosphate) are distributed separately intra-orally or delivered in a product with a low water activity. As this salt mixes with saliva, it releases calcium and phosphate ions. The combine effect of calcium ions with phosphate ions to produce an ion reactivity product for ACP that increases its solubility product results in the precipitation of ACP or, in the presence of fluoride ions, amorphous calcium fluoride phosphate (ACFP). (30)


VIII. Natural Remedies

Chewing sticks (miswaks) are used in many countries around the world for cleaning purposes, often up to 5 times a day. Fluoridated miswaks have a dual effect: producing a high fluoride concentration on the teeth and cleaning the buccal surfaces. (31)

Due to the innate proteolytic activity of enzymes, proteins are hydrolyzed, yielding peptides and amino acids. Enzymes have demonstrated reductions in plaque and gingivitis and they have the potential to lyse adhesive bonds between bacteria and the dental pellicle. Bromelain is a naturally occurring cysteine protease which is derived from pineapple stalks and is used in food and medical industries.32


Recent Advances

Fluoride-containing agents are used to increase remineralization, but the pleasing and esthetic appearance of enamel is usually not sufficiently enhanced. When treated with fixed appliances, sealants or bonding agents — are applied before or after the fixed appliances are bonded — as well as fluoride- or chlorhexidine containing mouthwashes as well as casein phosphor peptide amorphous calcium phosphate containing pastes (CPPACP) have been proposed. CPPACP-containing pastes or bioactive glasses can be used to enhance remineralization have been shown to enhance remineralization. The esthetic appearance most often remains impaired.

Microabrasion is another treatment option which is most suitable for very superficial lesions. In the case of deeper enamel lesions, concave tooth surfaces may result. Direct and indirect restorations also lead to predictable and satisfactory results, but these should be used in cavitated lesions. By resin infiltration, the microporous enamel areas of initial carious lesions are obturated by low-viscosity light-cured resins (infiltrants), thus, inhibiting further caries progression. Apart from caries inhibition, resin infiltration is also able to mask white spot lesions. As the refractive index of the infiltrant (1.52) is close to the index of enamel/apatite (1.62), as opposed to the indices of water (1.33) and air (1.00), light scattering is reduced with increasing degree of infiltration. (33)

Topical application of remineralizing agents, micro abrasion , and bleaching represent attempts to reverse enamel demineralization and/or to improve tooth appearance. The resin infiltration technique was also found to be useful in these cases. (34)

Resin infiltration is based on the hydrochloric acid erosion of the lesion surface and posterior infiltration of a low-viscosity resin into the intercristaline spaces of hypocalcified or demineralized enamel. This alters the refractive index (RI) of the porous enamel, formerly filled with air (RI=1.00) or water (RI=1.33), since the infiltrated resinous material shows a RI (1.52) closer to hydroxyapatite (1.62). As a consequence, the optical characteristics of the affected enamel are altered and it seems like the surrounding sound enamel (Marinelli G et al). The penetration of the low-viscosity resin into porous enamel with caries or hypomineralization has been shown in in vitro studies. [8-10] Additionally, color masking efficacy with resin infiltration has been demonstrated using artificial caries models. Some clinical reports also showed favorable esthetic results. Nevertheless, there is a lack of evidence concerning the clinical efficacy of the technique for camouflaging enamel whitish discolorations. (34)

Clinpro™ XT varnish showed significantly better improvement than Icon® resin infiltration in restoring the colour and lightness of the WSLs at 3 and 6 months. The fluorescence loss significantly recovered with both intervention methods between immediate application and at 6 months. However, Clinpro™ XT varnish-treated WSLs showed a statistically significant difference compared to the adjacent sound enamel at 6 months. (35)

The bioactive glass is deemed more effective against WSL development due to its ability to immediately repair enamel surface, low cytotoxicity, and high biocompatibility. Moreover, clinical studies on bioactive glass are still needed to determine its acceptability among patients with fixed orthodontic appliance.36

Previous studies showed that fluoride-containing glass ionomer and composite adhesives decrease enamel decalcification around orthodontic brackets. However, the fluoride containing and releasing adhesives and primers show their greatest levels during the initial weeks after bracket bonding. Therefore, a fluoride-containing dentifrices and materials would not have a significant preventive effect on enamel demineralization if it was used just once. Fluoride releasing materials should be applied repeatedly to increase their mode of action. (37)


WSL are a major side effect of orthodontic fixed treatments. The prevention and treatment of this problem is the goal of any orthodontist because untreated WSL can lead to the formation of dental caries. This review showed the use of various strategies in preventing WSLs during and after fixed orthodontic therapy. To conclude these lesions should be managed in the initial stages of treatment and patients should be educated about the importance of oral prophylaxis and maintaining good oral hygiene habits. Prophylaxis with topical fluorides, including high-fluoride toothpastes, fluoride mouthwashes, gels, varnishes, fluoride-containing bonding materials, and elastic ligatures (Maryam Khoroushi et al) is recommended. Recently, other materials and techniques are also implemented, including the application of casein phosphopeptides-amorphous calcium phosphate, antiseptics, probiotics, polyols, sealants, laser, tooth bleaching agents, resin infiltration, and microabrasion. (38)



1. Khoroushi M, Kachuie M. Prevention and treatment of white spot lesions in orthodontic patients. Contemporary clinical dentistry. 2017 Jan;8(1):11.

2. Gorelick L, Geiger AM, Gwinnett AJ. Incidence of white spot formation after bonding and banding. American journal of orthodontics. 1982 Feb 1;81(2):93-8.

3. Akin M, Basciftci FA. Can white spot lesions be treated effectively?. The Angle Orthodontist. 2012 Sep;82(5):770-5.

4. Behnan SM, Arruda AO, González-Cabezas C, Sohn W, Peters MC. In-vitro evaluation of various treatments to prevent demineralization next to orthodontic brackets. American Journal of Orthodontics and Dentofacial Orthopedics. 2010 Dec 1;138(6):712-e1.

5. Sudjalim TR, Woods MG, Manton DJ, Reynolds EC. Prevention of demineralization around orthodontic brackets in vitro. American journal of orthodontics and Dentofacial orthopedics. 2007 Jun 1;131(6):705-e1.

6. Shungin D, Olsson AI, Persson M. Orthodontic treatment-related white spot lesions: a 14-year prospective quantitative follow-up, including bonding material assessment. American Journal of Orthodontics and Dentofacial Orthopedics. 2010 Aug 1;138(2):136-e1.

7. Chen H, Liu X, Dai J, Jiang Z, Guo T, Ding Y. Effect of remineralizing agents on white spot lesions after orthodontic treatment: a systematic review. American journal of orthodontics and dentofacial orthopedics. 2013 Mar 1;143(3):376-82.

8. Maxfield BJ, Hamdan AM, Tüfekçi E, Shroff B, Best AM, Lindauer SJ. Development of white spot lesions during orthodontic treatment: perceptions of patients, parents, orthodontists, and general dentists. American Journal of Orthodontics and Dentofacial Orthopedics. 2012 Mar 1;141(3):337-44.

9. Øgaard B, Rølla G, Arends J, Ten Cate JM. Orthodontic appliances and enamel demineralization Part  Prevention and treatment of lesions. American Journal of Orthodontics and Dentofacial Orthopedics. 1988 Aug 1;94(2):123-8

10.  Sundararaj D, Venkatachalapathy S, Tandon A, Pereira A. Critical evaluation of incidence and prevalence of white spot lesions during fixed orthodontic appliance treatment: A meta-analysis. Journal of International Society of Preventive & Community Dentistry. 2015 Nov;5(6):433.

11.Gu X, Yang L, Yang D, Gao Y, Duan X, Zhu X, Yuan H, Li J. Esthetic improvements of postorthodontic white-spot lesions treated with resin infiltration and microabrasion: A split-mouth, randomized clinical trial. The Angle Orthodontist. 2019 May;89(3):372-7.

12.Gorton J, Featherstone JD. In vivo inhibition of demineralization around orthodontic brackets. American journal of orthodontics and dentofacial orthopedics. 2003 Jan 1;123(1):10-4.

13.Bishara SE, Ostby AW. White spot lesions: formation, prevention, and treatment. InSeminars in orthodontics 2008 Sep 1 (Vol. 14, No. 3, pp. 174-182). WB Saunders.

14.Chang HS, Walsh LJ, Freer TJ. Enamel demineralization during orthodontic treatment. Aetiology and prevention. Australian dental journal. 1997 Oct;42(5):322-7.

15.Oggard B. Prevalence of white spot lesions in 19-year-olds: a study on untreated and orthodontically treated persons 5 years treatment. Am J Orthod Dentofac Orthop. 1989; 96: 423-

16. Muntean A, Sava S, Delean AG, Mihailescu AM, Dumitrescu LS, Moldovan M, Festila DG. Toothpaste composition effect on enamel chromatic and morphological characteristics: In vitro analysis. Materials. 2019 Jan;12(16):2610.

17.Ballard RW, Hagan JL, Phaup AN, Sarkar N, Townsend JA, Armbruster PC. Evaluation of 3 commercially available materials for resolution of white spot lesions. American Journal of Orthodontics and Dentofacial Orthopedics. 2013 Apr 1;143(4):S78-84.

18. Marini I, Bortolotti F, Parenti SI, Gatto MR, Bonetti GA. Combined effects of repeated oral hygiene motivation and type of toothbrush on orthodontic patients: a blind randomized clinical trial. The Angle Orthodontist. 2014 Sep;84(5):896-901.

19. Acharya S, Goyal A, Utreja AK, Mohanty U. Effect of three different motivational techniques on oral hygiene and gingival health of patients undergoing multibracketed orthodontics. The Angle Orthodontist. 2011 Sep;81(5):884-8.

20.  Lale S, Solak H, H?nçal E, Vahdettin L. In Vitro Comparison of Fluoride, Magnesium, and Calcium Phosphate Materials on Prevention of White Spot Lesions around Orthodontic Brackets. BioMed research international. 2020 Mar 30;2020.

21. Kokich V. What's new in dentistry. The Angle Orthodontist. 1997 Oct;67(5):325-6.

22. Benson PE, Shah AA, Millett DT, Dyer F, Parkin N, Vine RS. Fluorides, orthodontics and demineralization: a systematic review. Journal of orthodontics. 2005 Jun;32(2):102-14.

23.  Kau CH, Wang J, Palombini A, Abou-Kheir N, Christou T. Effect of fluoride dentifrices on white spot lesions during orthodontic treatment: A randomized trial. The Angle Orthodontist. 2019 May;89(3):365-71.

24. Bailey DL, Adams GG, Tsao CE, Hyslop A, Escobar K, Manton DJ, Reynolds EC, Morgan MV. Regression of post-orthodontic lesions by a remineralizing cream. Journal of dental research. 2009 Dec;88(12):1148-53.

25. Reynolds EC. Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions. Journal of dental research. 1997 Sep;76(9):1587-95.

26. Beerens MW, Van Der Veen MH, Van Beek H, Ten Cate JM. Effects of casein phosphopeptide amorphous calcium fluoride phosphate paste on white spot lesions and dental plaque after orthodontic treatment: a 3?month follow?up. European journal of oral sciences. 2010 Dec;118(6):610-7.

27. Tschoppe P, Zandim DL, Martus P, Kielbassa AM. Enamel and dentine remineralization by nano-hydroxyapatite toothpastes. Journal of dentistry. 2011 Jun 1;39(6):430-7.

28. Cochrane NJ, Saranathan S, Cai F, Cross KJ, Reynolds EC. Enamel subsurface lesion remineralisation with casein phosphopeptide stabilised solutions of calcium, phosphate and fluoride. Caries research. 2008;42(2):88-97.

29.Cochrane NJ, Cai F, Huq NL, Burrow MF, Reynolds EC. New approaches to enhanced remineralization of tooth enamel. Journal of dental research. 2010 Nov;89(11):1187-97.

30. Baeshen HA, Lingström P, Birkhed D. Effect of fluoridated chewing sticks (Miswaks) on white spot lesions in postorthodontic patients. American Journal of Orthodontics and Dentofacial Orthopedics. 2011 Sep 1;140(3):291-7.

31. Rose J, Ghoneima A, Lippert F, Maxwell L, Eckert G, Stewart KT. A visual evaluation of oral plaque removal utilizing an adjunct enzyme pre-rinse in orthodontic subjects. The Angle Orthodontist. 2020 Nov 1;90(6):844-50.

32. Geiger AM, Gorelick L, Gwinnett AJ, Benson BJ. Reducing white spot lesions in orthodontic populations with fluoride rinsing. American Journal of Orthodontics and Dentofacial Orthopedics. 1992 May 1;101(5):403-7.

33. Bourouni S, Dritsas K, Kloukos D, Wierichs RJ. Efficacy of resin infiltration to mask post-orthodontic or non-post-orthodontic white spot lesions or fluorosis—A systematic review and meta-analysis. Clinical oral investigations. 2021 Aug;25(8):4711-9.

34. Marinelli G, Inchingolo AD, Inchingolo AM, Malcangi G, Limongelli L, Montenegro V, Coloccia G, Laudadio C, Patano A, Inchingolo F, Bordea IR. White spot lesions in orthodontics: prevention and treatment. A descriptive review. Journal of Biological Regulators and Homeostatic Agents. 2021 Mar 1;35(2 Suppl. 1):227-40.

35. Borges AB, Caneppele TM, Masterson D, Maia LC. Is resin infiltration an effective esthetic treatment for enamel development defects and white spot lesions? A systematic review. Journal of dentistry. 2017 Jan 1;56:11-8.

36. Kannan A, Padmanabhan S. Comparative evaluation of Icon® resin infiltration and Clinpro™ XT varnish on colour and fluorescence changes of white spot lesions: a randomized controlled trial. Progress in orthodontics. 2019 Dec;20(1):1-8.

37. Linjawi AI. Sealants and White Spot Lesions in Orthodontics: A Review. The Journal of Contemporary Dental Practice. 2020 Aug 19;21(7):808-14.

38. Comert S, Oz AA. Clinical effect of a fluoride-releasing and rechargeable primer in reducing white spot lesions during orthodontic treatment. American Journal of Orthodontics and Dentofacial Orthopedics. 2020 Jan 1;157(1):67-72.